Kucich U, Christner P, Weinbaum G, Rosenbloom J
Am Rev Respir Dis. 1980 Sep;122(3):461-5. doi: 10.1164/arrd.1980.122.3.461.
Pulmonary emphysema is a disease in which peptides formed by the enzymatic degradation of the amorphous component of interstitial elastin may be release from the lung. In order to provide a test that can monitor the destruction of lung elastin invivo, we developed a hemagglutination inhibiton assay that specifically and quantitatively measure elastin-derived peptides in serum. Using this method, was measured the concentration of elastin-derived peptides in the serums of dogs developing emphysema through the administration of porcine pancreatic elastase. Elastin-derived peptides could be detected in the serums for a period of 12 days after the administration of a single 25- or 50-mg dose of elastase, and for at least 40 days after a 100-mg dose. There was a good correlation between the maximal concentration of elastin derived peptides found in the serums and the amount of elastase administered to the animal. This immunologic method may be useful in following the progression of experiment emphysema.
肺气肿是一种疾病，其中由间质弹性蛋白无定形成分的酶促降解形成的肽可能从肺中释放出来。为了提供一种能够监测体内肺弹性蛋白破坏情况的检测方法，我们开发了一种血凝抑制试验，该试验可特异性地定量测量血清中弹性蛋白衍生的肽。使用这种方法，测量了通过给予猪胰弹性蛋白酶而患肺气肿的犬血清中弹性蛋白衍生肽的浓度。在单次给予25毫克或50毫克剂量的弹性蛋白酶后12天内，以及给予100毫克剂量后至少40天内，均可在血清中检测到弹性蛋白衍生肽。血清中发现的弹性蛋白衍生肽的最大浓度与给予动物的弹性蛋白酶量之间存在良好的相关性。这种免疫方法可能有助于追踪实验性肺气肿的进展。
