Kinawi A, Teller C
J Clin Chem Clin Biochem. 1978 Jul;16(7):413-9.
The construction and operation of a new dialysis device are described. The unbound fraction of a drug in a buffered solution of albumin can be determined after 10-15 minutes. The usefullness of this device was demonstrated by the determination of the unbound fraction of Carbamazepine or Promazine in buffered solutions of bovine serum albumin. The results agreed with those obtained by control methods (equilibrium dialysis and gel filtration). Furthermore, the binding of Carbamazepine to bovine serum albumin was characterised by determining the overall binding constant (K1), the apparent binding constant (k+), the number of binding sites per molecule of albumin (n), the free reaction energy deltaFdegrees, and the unbound fraction of the drug.
本文描述了一种新型透析装置的构建与运行。在白蛋白缓冲溶液中，10 - 15分钟后即可测定药物的未结合分数。通过测定牛血清白蛋白缓冲溶液中卡马西平或丙嗪的未结合分数，证明了该装置的实用性。结果与通过对照方法（平衡透析和凝胶过滤）获得的结果一致。此外，通过测定总结合常数（K1）、表观结合常数（k+）、每分子白蛋白的结合位点数（n）、自由反应能deltaFdegrees以及药物的未结合分数，对卡马西平与牛血清白蛋白的结合进行了表征。
