Cullen A P
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1980;214(2):107-18. doi: 10.1007/BF00572789.
A 5,000 W Xe-Hg high pressure lamp and a double monochromator were used to produce a 3.3 nm half-bandpass ultraviolet radiation at 295 nm. Pigmented rabbit eyes were irradiated with radiant exposures from 140 Jm-2 to 10,000 Jm-2 and evaluated by slit-lamp biomicroscopy, light and electron microscopy. Corneal threshold (Hc) was 200 Jm-2 and lens threshold (HL) was 7,500 Jm-2. The most repeatable and reliable corneal response to these levels of UV was the development of corneal epithelial granules. Histological changes included a loss of superficial epithelial cells and selective UV induced autolysis of the wing cells. It is suggested that the biomicroscopically observed granules are the clinical manifestation of the secondary lysosomes revealed by light and electron microscopy. It is proposed that UV breaks down the primary lysosome membranes to release hydrolytic enzymes which in turn form the secondary lysosomes during autolysis. Extreme levels of radiant exposure at 295 nm result in indiscriminate destruction of all layers of the corneal epithelium, but the posterior cornea was spared.
使用一台5000瓦的氙汞高压灯和一台双单色仪，产生波长为295纳米、半带宽为3.3纳米的紫外线辐射。用140焦每平方米至10000焦每平方米的辐射暴露量照射有色素的兔眼，并通过裂隙灯生物显微镜检查、光学显微镜和电子显微镜进行评估。角膜阈值（Hc）为200焦每平方米，晶状体阈值（HL）为7500焦每平方米。对这些紫外线水平最具重复性和可靠性的角膜反应是角膜上皮颗粒的形成。组织学变化包括表层上皮细胞的丢失以及紫外线诱导的翼状细胞选择性自溶。有人认为，生物显微镜下观察到的颗粒是光学显微镜和电子显微镜下显示的次级溶酶体的临床表现。有人提出，紫外线会破坏初级溶酶体膜，释放水解酶，这些水解酶在自溶过程中进而形成次级溶酶体。295纳米的极高辐射暴露水平会导致角膜上皮各层被无差别破坏，但角膜后部除外。
