Ip M P, Kleinerman J, Sorensen J
Exp Lung Res. 1980 Aug;1(3):181-9. doi: 10.3109/01902148009065458.
Intravenous injection of exogenous pancreatic elastase to hamsters within tolerable limits (up to 133 units/100 g animal) induced no significant changes in lung elastin as compared with the controls whereas intratracheal injection of a much smaller amount of enzyme (5 units) caused a remarkable decrease in total elastin within 24 hr. Decrease in lung collagen is observed by both routes of elastase injection only at the highest tolerable level of enzymes utilized (28 U IT and 133 U IV). The large differences in the tolerable limits of IV and IT injections of elastase may be ascribed to large differences in dilution volumes encountered in the vascular circuit as compared to the airways and to the nature and amounts of elastase inhibitors in serum as opposed to pulmonary fluid. Collagenase impurities in the elastase may also be in part responsible for the observed biochemical changes in lung collagen.
在可耐受限度内（每100克动物体重高达133单位）给仓鼠静脉注射外源性胰弹性蛋白酶，与对照组相比，肺弹性蛋白无显著变化；而气管内注射少量得多的酶（5单位）在24小时内导致总弹性蛋白显著减少。仅在使用的酶的最高可耐受水平（气管内注射28单位和静脉注射133单位）下，通过两种弹性蛋白酶注射途径均可观察到肺胶原蛋白减少。静脉注射和气管内注射弹性蛋白酶的可耐受限度存在巨大差异，这可能归因于与气道相比，血管回路中的稀释体积存在巨大差异，以及血清中弹性蛋白酶抑制剂的性质和数量与肺液不同。弹性蛋白酶中的胶原酶杂质也可能部分导致了观察到的肺胶原蛋白的生化变化。
