Fay D, Bowman B U
J Virol. 1978 Aug;27(2):432-5. doi: 10.1128/JVI.27.2.432-435.1978.
The morphologies of native and chloroform-methanol-treated mycobacteriophage R1 were compared by electron microscopy, utilizing three negative stains. R1 was determined to be a complex phage. The head appears as an elongated cylinder with a pointed end (93 +/- 3 by 42 +/- 3 nm) constructed from an orderly arrangement of capsomeres. The phage tail measures 209 +/- 11 by 11 +/- 1 nm and possesses a striated surface with two base plates at its distal end. Treatment of R1 with chloroform-methanol resulted in disruption of both the head and tail structures and was accompanied by loss of infectivity. However, because no likely lipid-containing structure was observed in native phages, there is the possibility that the mechanism of chloroform-methanol inactivation is something other than lipid extraction.
利用三种负染色法，通过电子显微镜比较了天然的和经氯仿-甲醇处理的分枝杆菌噬菌体R1的形态。R1被确定为一种复合噬菌体。头部呈一端尖锐的细长圆柱体（93±3×42±3纳米），由有序排列的衣壳粒构成。噬菌体尾部尺寸为209±11×11±1纳米，表面有条纹，远端有两个基板。用氯仿-甲醇处理R1导致头部和尾部结构均被破坏，并伴随着感染力丧失。然而，由于在天然噬菌体中未观察到可能含脂质的结构，氯仿-甲醇灭活机制有可能不是脂质提取。
