Durda P J, Pagano R J, Bauman N, Brockman J A
J Allergy Clin Immunol. 1982 Nov;70(5):353-60. doi: 10.1016/0091-6749(82)90024-0.
The effects of iodipamide on C3 and factor B in normal human serum and in purified form have been examined by immunoelectrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Temperature-dependent changes in immunoelectrophoretic profiles have been observed; however, these are not the same as those obtained after treatment of normal human serum (NHS) with cobra venom factor Naja naja. Analyses of iodipamide-treated NHS and purified C3 and factor B by reducing SDS-PAGE indicate that no macromolecular changes have occurred in C3 and factor B that can be ascribed to proteolysis (i.e., activation). The changes observed in C3 and factor B, including loss of hemolytic activity, appear to be due to direct interactions between iodipamide and C3 and factor B. In the case of factor B, iodipamide treatment at 37 degrees C induces aggregation, which is reversible upon reduction with beta-mercaptoethanol.
已通过免疫电泳和十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）研究了碘番酸对正常人血清及纯化形式的C3和B因子的影响。观察到免疫电泳图谱随温度变化；然而，这些变化与用眼镜蛇毒因子（眼镜蛇）处理正常人血清（NHS）后获得的变化不同。通过还原SDS-PAGE对经碘番酸处理的NHS以及纯化的C3和B因子进行分析表明，C3和B因子未发生可归因于蛋白水解（即激活）的大分子变化。在C3和B因子中观察到的变化，包括溶血活性丧失，似乎是由于碘番酸与C3和B因子之间的直接相互作用所致。就B因子而言，在37℃下用碘番酸处理会诱导聚集，用β-巯基乙醇还原后这种聚集是可逆的。
