Hasselbacher P, Hahn J
J Allergy Clin Immunol. 1980 Sep;66(3):217-22. doi: 10.1016/0091-6749(80)90042-1.
The effect of several radiographic contrast media on the complement system in vitro was examined using quantitative crossed immunoelectrophoresis. Diatrizoate, iothalamate, metrizoate, and methiodal did not cause electrophoretic conversion of C3 or properdin factor B. In fact, the small degree of spontaneous conversion occurring when serum is incubated in polypropylene tubes was inhibited by these contrast media or by hypertonic sodium chloride. Metrizamide caused enhanced conversion of C3 and factor B that was only partially inhibited by ethylene glycol tetracetic acid (EGTA) but completely inhibited by ethylenediaminetetraacetic acid (EDTA). Iodipamide caused a unique electrophoretic alteration of C3 that was not affected by EDTA or several other inhibitors and that was not identical with the usual products of C3 activation. Iodipamide in very low concentrations inhibited complement activation through the classical pathway.
采用定量交叉免疫电泳法检测了几种放射造影剂在体外对补体系统的影响。泛影葡胺、碘他拉酸盐、甲泛葡胺和甲碘吡酮均未引起C3或备解素因子B的电泳转化。事实上，当血清在聚丙烯管中孵育时发生的少量自发转化受到这些造影剂或高渗氯化钠的抑制。甲泛影酰胺引起C3和因子B的转化增强，仅部分受乙二醇四乙酸（EGTA）抑制，但完全受乙二胺四乙酸（EDTA）抑制。碘番酸引起C3独特的电泳改变，不受EDTA或其他几种抑制剂的影响，且与C3活化的常见产物不同。极低浓度的碘番酸可抑制经典途径的补体激活。
