一个包含天冬氨酸tRNA、甘氨酸tRNA、谷氨酸tRNA基因及假基因的大鼠tRNA基因簇的特征分析
Characterization of a rat tRNA gene cluster containing the genes for tRNAAsp, tRNAGly, and tRNAGlu, and pseudogenes.
作者信息
Sekiya T, Noguchi S, Shibuya K, Kuchino Y, Nishimura S
出版信息
Princess Takamatsu Symp. 1982;12:53-62.
Molecular cloning and sequence analysis of a rat DNA fragment hybridized to purified tRNAAsp revealed that the genes for tRNAAspGAU(C), tRNAGlyGGA(G), and tRNAGluGAG are in a cluster on the rat chromosome. These genes are arranged in this order and are regularly separated by DNA regions of about 450 bp. The cluster is reiterated about 10 times on the haploid DNA and is present exclusively in a 3.3 kb region cleaved by restriction endonuclease EcoRI. Five lambda clones each containing an independent repeating unit were isolated from a rat gene library. The clones revealed that the length of the repeating unit including the 3.3 kb EcoRI fragment was at least 13.5 kb. Nucleotide sequence analysis and in vitro transcription of the 3.3 kb DNA in the isolated lambda clones showed sequence variations among the repeating units and the presence of incomplete genes for tRNAGly and tRNAGlu within the clusters.
与纯化的天冬氨酸tRNA杂交的大鼠DNA片段的分子克隆及序列分析表明，天冬氨酸tRNA（GAU(C)）、甘氨酸tRNA（GGA(G)）和谷氨酸tRNA（GAG）的基因在大鼠染色体上成簇排列。这些基因按此顺序排列，且被约450 bp的DNA区域规则隔开。该基因簇在单倍体DNA上重复约10次，且仅存在于被限制性内切酶EcoRI切割的3.3 kb区域。从大鼠基因文库中分离出五个λ克隆，每个克隆都包含一个独立的重复单元。这些克隆表明，包括3.3 kb EcoRI片段在内的重复单元长度至少为13.5 kb。对分离出的λ克隆中3.3 kb DNA的核苷酸序列分析及体外转录显示，重复单元之间存在序列变异，且基因簇内存在甘氨酸tRNA和谷氨酸tRNA的不完整基因。
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