Dugal R, Massé R, Sanchez G, Bertrand M J
J Anal Toxicol. 1980 Jan-Feb;4(1):1-12. doi: 10.1093/jat/4.1.1.
This paper presents the methodological aspects of a computerized system for the gas-chromatographic screening and primary identification of central nervous system stimulants and narcotic analgesics (including some of their respective metabolites) extracted from urine. The operating conditions of a selective nitrogen detector for optimized analytical functions are discussed, particularly the effect of carrier and fuel gas on the detector's sensitivity to nitrogen-containing molecules and discriminating performance toward biological matrix interferences. Application of simple extraction techniques, combined with rapid derivatization procedures, computer data acquisition, and reduction of chromatographic data are presented. Results show that this system approach allows for the screening of several drugs and their metabolites in a short amount of time. The reliability and stability of the system have been tested by analyzing several thousand samples for doping control at major international sporting events and for monitoring drug intake in addicts participating in a rehabilitation program. Results indicate that these techniques can be used and adapted to many different analytical toxicology situations.
本文介绍了一种计算机化系统的方法学方面，该系统用于对从尿液中提取的中枢神经系统兴奋剂和麻醉性镇痛药（包括它们各自的一些代谢物）进行气相色谱筛选和初步鉴定。讨论了用于优化分析功能的选择性氮检测器的操作条件，特别是载气和燃气对检测器对含氮分子的灵敏度以及对生物基质干扰的区分性能的影响。介绍了简单提取技术与快速衍生化程序、计算机数据采集以及色谱数据简化的结合应用。结果表明，这种系统方法能够在短时间内对多种药物及其代谢物进行筛选。该系统的可靠性和稳定性已通过分析数千个样本进行测试，这些样本用于重大国际体育赛事的兴奋剂控制以及监测参与康复计划的成瘾者的药物摄入情况。结果表明，这些技术可用于并适用于许多不同的分析毒理学情况。
