Beall P T
Magn Reson Imaging. 1982;1(3):165-79. doi: 10.1016/0730-725x(82)90208-9.
The ability of NMR parameters T1 and T2 to be used to distinguish one tissue from another and diseased from normal tissues has wide application in diagnostic medicine. Measurement of such values in vitro on excised tissues and fluids was the basis for fundamental discoveries which provided the impetus for the development of in vivo clinical whole body NMR imagers. Therefore, as in vivo imaging grows, the need for screening and testing of new ideas in vitro will grow. The success of data collection in vitro depends greatly on the care and understanding with which biological samples are processed. This paper summarizes proven methods for handling of soft and firm biopsy material, blood components, body fluids, and culture cells. The effects of temperature variation and storage parameters are discussed for animal and human samples. The paper is a practical guide to the hows and whys of biological sample handling for NMR.
核磁共振参数T1和T2用于区分不同组织以及病变组织与正常组织的能力在诊断医学中有着广泛应用。在体外对切除的组织和液体进行此类值的测量是一些基础发现的基础，这些发现为体内临床全身核磁共振成像仪的发展提供了动力。因此，随着体内成像的发展，体外筛选和测试新想法的需求也会增加。体外数据收集的成功在很大程度上取决于处理生物样本时的细心程度和理解程度。本文总结了处理软质和硬质活检材料、血液成分、体液及培养细胞的成熟方法。讨论了温度变化和储存参数对动物和人类样本的影响。本文是一份关于核磁共振生物样本处理方法及原因的实用指南。
