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[肿瘤患者血清中的循环免疫复合物]

[Circulating immune complexes in the serum of tumor patients].

作者信息

Alvarez R, García Gómez A, Subiza J L, Domínguez J, Bootello A, Coll J

出版信息

Rev Esp Oncol. 1982;29(4):727-34.

PMID:6927370
Abstract

The conditions for the detection of circulating immune complexes (CIC) were established by combination of polyethylene glycol precipitation with a nephelometric determination on the precipitate of endogenous C1q. The CIC precipitated from the sera of patients with breast carcinoma, malignant melanoma or autoimmune diseases were dissociated in SDS-beta-mercaptoethanol buffer and no differences were observed in the electrophoretic patterns os polyacrylamide gels allowing to characterize tumour specific antigens.

摘要

通过聚乙二醇沉淀法与对内源性C1q沉淀进行比浊测定相结合,建立了循环免疫复合物(CIC)的检测条件。从乳腺癌、恶性黑色素瘤或自身免疫性疾病患者血清中沉淀出的CIC在SDS-巯基乙醇缓冲液中解离,聚丙烯酰胺凝胶电泳图谱未观察到差异,从而得以鉴定肿瘤特异性抗原。

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