Alvarez R, García Gómez A, Subiza J L, Domínguez J, Bootello A, Coll J
Rev Esp Oncol. 1982;29(4):727-34.
The conditions for the detection of circulating immune complexes (CIC) were established by combination of polyethylene glycol precipitation with a nephelometric determination on the precipitate of endogenous C1q. The CIC precipitated from the sera of patients with breast carcinoma, malignant melanoma or autoimmune diseases were dissociated in SDS-beta-mercaptoethanol buffer and no differences were observed in the electrophoretic patterns os polyacrylamide gels allowing to characterize tumour specific antigens.
通过聚乙二醇沉淀法与对内源性C1q沉淀进行比浊测定相结合,建立了循环免疫复合物(CIC)的检测条件。从乳腺癌、恶性黑色素瘤或自身免疫性疾病患者血清中沉淀出的CIC在SDS-巯基乙醇缓冲液中解离,聚丙烯酰胺凝胶电泳图谱未观察到差异,从而得以鉴定肿瘤特异性抗原。