Toxicity of 29 plasticizers to HeLa cells in the MIT-24 system.

The toxicity to HeLa cells of 29 plasticizers was determined in the MIT-24 test system. The 7-day IC50 for HeLa cells varied from 260 to 1.5 g/l. Phthalates, adipates, sebacates, azelates and phosphates with long carbon chain alcohols were very non-toxic to the cells, probably due to insolubility in water of the compounds, while the citrates, some phosphates and the 2 polymer plasticizers had a higher toxicity to the cells. A comparison of the HeLa cytotoxicity with the toxicity in vitro to other cells for 7 plasticizers showed a similarity of the cytotoxicity to all cell types. A comparison of the HeLa cytotoxicity for 20 plasticizers with i.p. lethal dosage in rodents demonstrated a rough similarity of values, suggesting a toxicity in rodents of the compounds by toxic interference of the agents with basal functions and structures of tissues (basal cytotoxicity). Tissue culture studies of the cytotoxic mechanisms of the plasticizers therefore could reveal modes of toxic action in vivo.