Schwenk H U, Schneider U, Herzog K H
Blut. 1980 Jan;40(1):7-15. doi: 10.1007/BF01028359.
The binding of 12 different fluorescein-conjugated lectins to 10 ALL (acute lymphoblastic leukemia) cell lines and two cell lines derived from patients in blast crisis of myeloid leukemia was examined. The specificity of the membrane fluorescence was demonstrated by inhibition with various saccharides. Three of the lectins bound to all cell lines, four bound to only some of the lines, and five were not bound. There was no correlation between the binding pattern and the immunological phenotype of the cultured lymphoblasts. The lectin from Lens culinaris, however, in the experimental condition used (incubation at 4 degrees C, fluorescein conjugation at pH 8.5), bound only to the cell membranes of 'Ia-like antigen, positive cell lines. Although three lectins (Lens culinaris, Pisum sativum, concanavalin A) had an identical monosaccharide specificity, they bound to different cell lines. Membrane fluorescence with the lectins from Helix pomatia, Arachis hypogea, and Ricinus communis (MW 60,000) was achieved after treatment with neuraminidase. It was shown that binding of the lectins from Helix pomatia and Ricinus communis 60 was effected by enzymatically exposed glycoproteins, whereas the lectin Arachis hypogea was bound via neuraminidase which stuck to the cell membrane.
研究了12种不同的荧光素偶联凝集素与10种急性淋巴细胞白血病（ALL）细胞系以及2种来自髓系白血病原始细胞危象患者的细胞系的结合情况。通过用各种糖类进行抑制来证明膜荧光的特异性。其中3种凝集素与所有细胞系结合，4种仅与部分细胞系结合，5种不结合。培养的淋巴母细胞的结合模式与免疫表型之间没有相关性。然而，在所用的实验条件下（4℃孵育，pH 8.5进行荧光素偶联），来自小扁豆的凝集素仅与“Ia样抗原阳性细胞系的细胞膜结合。尽管3种凝集素（小扁豆凝集素、豌豆凝集素、伴刀豆球蛋白A）具有相同的单糖特异性，但它们与不同的细胞系结合。在用神经氨酸酶处理后，获得了与来自苹果蜗牛、花生和蓖麻（分子量60,000）的凝集素的膜荧光。结果表明，来自苹果蜗牛和蓖麻60的凝集素的结合是由酶促暴露的糖蛋白介导的，而花生凝集素是通过粘附在细胞膜上的神经氨酸酶结合的。
