Brown J M, Twentyman P R, Zamvil S S
J Natl Cancer Inst. 1980 Mar;64(3):605-11.
The radiation response of logarithmic growth phase and fed plateau phase RIF-1 cells in vitro was found to be characterized by D0 (slope of cell survival curve on semilog plot) values of 110 and 133 rads and extrapolation numbers of 36 and 28, respectively. The response of the tumor in vivo to X-irradiation in nonanesthetized mice showed a dependence on the tumor implantation site. In the leg muscle, the response indicated that most cells were at an intermediate level of oxygenation, whereas in the subcutaneous tissue of the flank, the response of the tumor indicated that it had a small fraction (1.5%) of hypoxic cells of maximum radioresistance. Misonidazole radiosensitized the leg-implanted tumor as measured both by cell survival and regrowth delay. The median tumor cure dose of radiation in mice was 3,990 rads (3,670--4,340), which agreed closely with that predicted from the radiation survival curve. The tumor was relatively insensitive to a single dose of 1,3-bis(2-chloroethyl)-1-nitrosourea, sensitive to a single dose of cis-platinum, and highly sensitive to a single dose of cyclophosphamide.
体外对数生长期和营养丰富平台期的RIF-1细胞的辐射反应表现为，其D0值（半对数图上细胞存活曲线的斜率）分别为110和133拉德，外推数分别为36和28。在未麻醉小鼠体内，肿瘤对X射线照射的反应取决于肿瘤植入部位。在腿部肌肉中，反应表明大多数细胞处于中等氧合水平，而在侧腹皮下组织中，肿瘤的反应表明其有一小部分（1.5%）具有最大放射抗性的缺氧细胞。用细胞存活和再生长延迟来衡量，灭滴灵使腿部植入的肿瘤产生放射增敏作用。小鼠辐射的中位肿瘤治愈剂量为3990拉德（3670 - 4340），这与从辐射存活曲线预测的值非常一致。该肿瘤对单剂量的1,3 - 双（2 - 氯乙基）- 1 - 亚硝基脲相对不敏感，对单剂量的顺铂敏感，对单剂量的环磷酰胺高度敏感。
