一氧化碳血蓝蛋白的发光
Luminescence of carbon monoxide hemocyanins.
作者信息
Kuiper H A, Agrò A F, Antonini E, Brunori M
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2387-9. doi: 10.1073/pnas.77.5.2387.
Abstract
The effect of carbon monoxide on the luminescence properties of Helix pomatia alpha-hemocyanin and Panulirus interruptus hemocyanin has been studied. These proteins, when saturated with carbon monoxide, show, besides the intrinsic fluorescence arising from the aromatic amino acid residues, emission in the visible region with a maximum between 540 and 560 nm. Results of carbon monoxide titration experiments and data from absorption and excitation-emission spectra provide convincing evidence that the observed emission originates from a fluorescent copper(I)-carbon monoxide complex, and this emission is interpreted as charge-transfer luminescence.
摘要
一氧化碳对罗马蜗牛α-血蓝蛋白和断沟龙虾血蓝蛋白发光特性的影响已得到研究。这些蛋白质在一氧化碳饱和时，除了由芳香族氨基酸残基产生的固有荧光外，在可见光区域还会出现发射峰，最大发射波长在540至560纳米之间。一氧化碳滴定实验结果以及吸收光谱和激发-发射光谱数据提供了令人信服的证据，表明观察到的发射源自荧光铜（I）-一氧化碳络合物，并且这种发射被解释为电荷转移发光。
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