Enzymic synthesis of steroid sulphates. XIII. Isolation and properties of dehydroepiandrosterone sulphotransferase from human foetal adrenals.

Human foetal adrenals have provided a rich source of steroid alcohol sulphotransferase (EC 2.8.2.-). The latter was isolated in pure form in one step by affinity chromatography on an (NH4)2SO4 cut derived from the cytosol fraction of the glands. The yield was 6-fold higher than that obtained from adult human adrenals. General properties of the enzyme are given and it appears to be identical to that obtained previously from adult human adrenals.