Nanci A, Babaï F, Dumont A
J Natl Cancer Inst. 1980 Dec;65(6):1273-83.
The response of peritoneal cells to transplantable Novikoff hepatoma was studied in immunized and nonimmunized inbred male SD rats to elucidate the role of macrophages in syngeneic tumor rejection. Specific immunization was induced by double sc challenge with viable tumor cells. Peritoneal cells were harvested by lavage at intervals of 6 hours and 1, 2, 3, 4, and 9 days after ip tumor injection. Some of the lavage fluid was used to determine the total number of cells, and the remainder was prepared for ultrastructural study. Different counts were made at the electron microscope level, and the ultrastructures of macrophages and tumor cells were studied. The results indicated that during the early intervals after tumor injection, the numbers of macrophages and lymphocytes were significantly increased in immunized animals. The mononuclear cells accumulated more slowly in the peritoneal cavities of nonimmunized rats. Morphologic heterogeneity was observed in the macrophage population of immunized rats up to day 4. Concurrent with these changes in macrophages were the decreased mitotic activity and ultrastructural alterations in tumor cells followed by their complete disappearance. In nonimmunized animals tumor cells proliferated actively and by day 9 constituted 85% of the peritoneal population.
为了阐明巨噬细胞在同基因肿瘤排斥反应中的作用，研究了免疫和未免疫的近交系雄性SD大鼠腹膜细胞对可移植的诺维科夫肝癌的反应。通过用活肿瘤细胞进行两次皮下攻击诱导特异性免疫。在腹腔注射肿瘤后6小时以及1、2、3、4和9天，每隔一段时间通过灌洗收集腹膜细胞。部分灌洗液用于确定细胞总数，其余部分则准备用于超微结构研究。在电子显微镜水平进行不同计数，并研究巨噬细胞和肿瘤细胞的超微结构。结果表明，在肿瘤注射后的早期，免疫动物体内巨噬细胞和淋巴细胞的数量显著增加。单核细胞在未免疫大鼠的腹腔中积累得更慢。直到第4天，在免疫大鼠的巨噬细胞群体中观察到形态学异质性。与巨噬细胞的这些变化同时发生的是肿瘤细胞有丝分裂活性降低和超微结构改变，随后肿瘤细胞完全消失。在未免疫动物中，肿瘤细胞活跃增殖，到第9天占腹膜细胞群体的85%。
