来自盘基网柄菌的单体肌动蛋白和丝状肌动蛋白的ATP驱动稳态交换
ATP-driven steady-state exchange of monomeric and filamentous actin from Dictyostelium discoideum.
作者信息
Simpson P A, Spudich J A
出版信息
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4610-3. doi: 10.1073/pnas.77.8.4610.
Abstract
When [35S]actin monomer from the slime mold Dictyostelium discoideum is added in trace amounts to a population of unlabeled Dictyostelium actin molecules assembled to steady state, it rapidly exchanges with the pool of actin filaments. This exchange between monomeric and filamentous actin is dependent on the presence of ATP. In addition, the exchange appears to occur via filament ends, because cytochalasin D, a drug that interacts specifically with actin filament ends to inhibit filament assenbly, inhibits the exchange reaction.
摘要
当将来自盘基网柄菌的[35S]肌动蛋白单体以痕量添加到组装至稳态的未标记盘基网柄菌肌动蛋白分子群体中时，它会迅速与肌动蛋白丝池进行交换。单体肌动蛋白和丝状肌动蛋白之间的这种交换依赖于ATP的存在。此外，这种交换似乎是通过丝端发生的，因为细胞松弛素D是一种与肌动蛋白丝端特异性相互作用以抑制丝组装的药物，它会抑制交换反应。
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POLYMERIZATION OF ACTIN FREE FROM NUCLEOTIDES AND DIVALENT CATIONS.无核苷酸和二价阳离子的肌动蛋白聚合作用
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Cytochalasin inhibits the rate of elongation of actin filament fragments.细胞松弛素抑制肌动蛋白丝片段的伸长速率。
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