Tauber R, Reutter W
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5282-6. doi: 10.1073/pnas.77.9.5282.
The interference of microtubular disruptors with the uptake of amino acids and other low molecular weight substrates has been studied in Morris hepatomas, host liver, and regenerating liver. Colchicine inhibits amino acid transport (alpha-aminoisobutyric acid, L-methionine, and L-leucine) in hepatomas by 59-98% whereas transport in host and regenerating liver is not impeded but increased. In hepatomas, treatment urea, and carbonate. Vinblastine, but not lumicolchicine or cytochalasin B, is an effective inhibitor. The inhibition of uptake is not linked to a decrease of cellular ATP and UTP. The data suggest that the transport of low molecular weight substrates in hepatomas is related to microtubules or other colchicine-binding structures, e.g., of the plasma membrane. This colchicine-sensitive uptake system in hepatomas may be due to the malignant transformation of hepatocytes.
已在莫里斯肝癌、宿主肝脏和再生肝脏中研究了微管破坏剂对氨基酸及其他低分子量底物摄取的干扰作用。秋水仙碱可使肝癌中氨基酸转运（α-氨基异丁酸、L-甲硫氨酸和L-亮氨酸）受到59% - 98%的抑制，而宿主肝脏和再生肝脏中的转运并未受阻，反而有所增加。在肝癌中，尿素和碳酸盐可作为有效抑制剂。长春花碱是一种有效抑制剂，而光秋水仙碱或细胞松弛素B则不是。摄取的抑制与细胞ATP和UTP的减少无关。数据表明，肝癌中低分子量底物的转运与微管或其他秋水仙碱结合结构（如质膜）有关。肝癌中这种对秋水仙碱敏感的摄取系统可能是由于肝细胞的恶性转化所致。
