Temperature-sensitive mutants of BALB/3T3 cells. III. Hybrids between ts2 and other mouse mutant cells affected in DNA synthesis and correction of ts2 defect by human X chromosome.

Complementation studies were performed with ts2, a mouse 3T3 cell mutant temperature sensitive (ts) for cell and viral DNA synthesis. The ts phenotype is corrected by non-ts mouse or human cells and a non-DNA ts mutant. This gene had been localized to a region on the human X chromosome near the HPRT locus based on isozyme and karyotype analysis of hybrids. Unusually rapid loss and fragmentation of human chromosomes occurs in hybrids with ts2. Hybrids between ts2 and other DNA- ts mutants of mouse cells did not show complementation of the growth phenotype.