人类红细胞葡萄糖转运蛋白的免疫学鉴定
Immunological identification of the human erythrocyte glucose transporter.
作者信息
Sogin D C, Hinkle P C
出版信息
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5725-9. doi: 10.1073/pnas.77.10.5725.
Abstract
A rabbit antibody against the human erythrocyte glucose transporter was purified by affinity chromatography and used to determine the distribution of transporter on polyacrylamide gels after electrophoresis in sodium dodecyl sulfate. Fresh erythrocyte ghosts showed transporter only at the broad 55,000 Mr band, as did the isolated transporter. HeLa cell plasma membranes showed a similar band of crossreacting material at Mr 55,000. The amount of crossreacting material in human erythrocyte ghosts and in plasma membranes from human HeLa cells and mouse L-1210 cells was determined in an enzyme-linked immunosorbent assay which gave results consistent with the extent of glucose-reversible binding of cytochalasin B.
摘要
一种针对人红细胞葡萄糖转运蛋白的兔抗体通过亲和层析进行纯化，并用于在十二烷基硫酸钠中电泳后测定聚丙烯酰胺凝胶上转运蛋白的分布。新鲜红细胞血影显示转运蛋白仅出现在宽的55,000 Mr条带处，分离出的转运蛋白也是如此。HeLa细胞质膜在Mr 55,000处显示出一条类似的交叉反应物质条带。在酶联免疫吸附测定中测定了人红细胞血影以及人HeLa细胞和小鼠L-1210细胞质膜中交叉反应物质的量，其结果与细胞松弛素B的葡萄糖可逆结合程度一致。
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