胎膜不参与皮质醇代谢的证据：双绒毛膜双胎妊娠研究

Evidence that fetal membranes are not involved in cortisol metabolism: study of dichorionic twin pregnancies.

作者信息

López Bernal A, Anderson A B, Parry D M, Turnbull A C

出版信息

Am J Obstet Gynecol. 1980 Dec 15;138(8):1168-72. doi: 10.1016/s0002-9378(16)32786-7.

Abstract

11 beta-Hydroxysteroid dehydrogenase (11 beta-HSD, E.C.1.1.1.146) activity has been measured in samples of decidua, choriodecidua, and chorion obtained from five sets of dichorionic twins, with radiolabeled cortisol and cortisone used as substrates. 11 beta-HSD activity was significantly higher in the decidua than in the choriodecidua, in both the oxidative (cortisol to cortisone) are reductive (cortisone to cortisol) directions (activity expressed as percentage conversion): 53.4% +/- 6.7% versus 38.5% +/- 7.4% (p < 0.05) and 21.3% +/- 2.2% versus 15.3% +/- 3.2% (p < 0.025) (mean +/- SEM, n = 10). No measurable activity in either direction was found in the chorion obtained from the wall separating the two amniotic sacs (decidua-free chorion). In both decidua and choriodecidua oxidative activity was higher than reductive activity (p < 0.005 for both). These results demonstrate that 11 beta-HSD activity attributed to the chorion is due to decidual contamination.

摘要

采用放射性标记的皮质醇和可的松作为底物，测定了从五组双绒毛膜双胎获取的蜕膜、绒毛蜕膜和绒毛膜样本中的11β-羟基类固醇脱氢酶（11β-HSD，E.C.1.1.1.146）活性。在氧化方向（皮质醇转化为可的松）和还原方向（可的松转化为皮质醇），蜕膜中的11β-HSD活性均显著高于绒毛蜕膜（活性以转化率百分比表示）：分别为53.4%±6.7% 对38.5%±7.4%（p<0.05）和21.3%±2.2%对15.3%±3.2%（p<0.025）（均值±标准误，n=10）。在分隔两个羊膜囊的壁上获取的绒毛膜（无蜕膜绒毛膜）中，未发现任何一个方向的可测量活性。在蜕膜和绒毛蜕膜中，氧化活性均高于还原活性（两者p<0.005）。这些结果表明，归因于绒毛膜的11β-HSD活性是由于蜕膜污染所致。