Kunke K S, Strunk R C
J Natl Cancer Inst. 1981 Jan;66(1):141-6.
In vitro production of the second and fourth components of complement (C2 and C4, respectively) by peritoneal macrophages from noninbred Hartley guinea pigs was tested after the animals had been inoculated with known carcinogens. The system demonstrated the capacity of N-nitrosodimethylamine to decrease C2 and C4 production. However, a similar decrease in C2 and C4 production was seen with only CCl4, 1 of the 10 chemical carcinogens studied. This system had little usefulness as a short-term screening procedure for the detection of carcinogenicity. The effect of the carcinogens on several other functions of peritoneal macrophages was also determined. The number of peritoneal exudate cells (PEC) was significantly lower in carcinogen-inoculated animals than in solvent-inoculated controls for three carcinogens: BeSO4, P < 0.005; CHCl3, P < 0.025; and CCl4, P < 0.01. However, the capacity of the PEC to adhere to plastic was decreased by only CHCl3 (P < 0.05), and adherent cells from all guinea pigs produced normal amounts of total secreted protein.
在非近交系哈特利豚鼠接种已知致癌物后，检测其腹腔巨噬细胞体外产生补体第二和第四成分（分别为C2和C4）的情况。该系统显示N-亚硝基二甲胺有降低C2和C4产生的能力。然而，在所研究的10种化学致癌物中，只有四氯化碳能使C2和C4产生出现类似下降。该系统作为检测致癌性的短期筛选程序几乎没有用处。还测定了致癌物对腹腔巨噬细胞其他几种功能的影响。对于三种致癌物，接种致癌物的动物腹腔渗出细胞（PEC）数量显著低于接种溶剂的对照组：硫酸铍，P<0.005；三氯甲烷，P<0.025；四氯化碳，P<0.01。然而，只有三氯甲烷能降低PEC黏附塑料的能力（P<0.05），并且所有豚鼠的黏附细胞产生的总分泌蛋白量正常。
