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能量转移诱导的特异性光亲和标记：应用于乙酰胆碱酯酶的不可逆抑制

Specific photoaffinity labeling induced by energy transfer: application to irreversible inhibition of acetylcholinesterase.

作者信息

Goeldner M P, Hirth C G

出版信息

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6439-42. doi: 10.1073/pnas.77.11.6439.

DOI:10.1073/pnas.77.11.6439
PMID:6935657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350300/
Abstract

p-Dimethylaminobenzene diazonium fluoroborate belongs to a class of potential photoaffinity labeling reagents which, by irradiation, produces a highly reactive electrophilic species. In addition, it can be photodecomposed by photoexcited tryptophan derivatives (e.g., N-acetyltryptophanamide and tryptophan residues belonging to acetylcholinesterase) by an energy transfer reaction. This substance is a competitive inhibitor of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) and is able to inactivate the enzyme either by photoaffinity labeling after irradiation at 410 nm or by an energy transfer reaction after irradiation at 295 nm. The efficiency of this method is demonstrated by an increase of the rate of enzyme inactivation as well as by a decrease of nonselective labeling with a radioactive inhibitor p-[methyl-3H]-dimethylaminobenzene diazonium fluoroborate.

摘要

对二甲氨基苯重氮氟硼酸盐属于一类潜在的光亲和标记试剂，通过照射可产生高活性的亲电物质。此外，它可通过能量转移反应被光激发的色氨酸衍生物（如N - 乙酰色氨酸酰胺和属于乙酰胆碱酯酶的色氨酸残基）光分解。该物质是乙酰胆碱酯酶（乙酰胆碱乙酰水解酶，EC 3.1.1.7）的竞争性抑制剂，能够通过在410 nm照射后的光亲和标记或在295 nm照射后的能量转移反应使酶失活。酶失活速率的增加以及放射性抑制剂对 - [甲基 - ³H] - 二甲氨基苯重氮氟硼酸盐非选择性标记的减少证明了该方法的有效性。

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Specific photoaffinity labeling induced by energy transfer: application to irreversible inhibition of acetylcholinesterase.能量转移诱导的特异性光亲和标记：应用于乙酰胆碱酯酶的不可逆抑制
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本文引用的文献

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ACETYLCHOLINESTERASE: STRUCTURAL REQUIREMENTS FOR BLOCKING DEACETYLATION.乙酰胆碱酯酶：阻断去乙酰化的结构要求
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Affinity chromatography of acetylcholinesterase. The importance of hydrophobic interactions.乙酰胆碱酯酶的亲和层析。疏水相互作用的重要性。
Eur J Biochem. 1976 Sep 15;68(2):531-9. doi: 10.1111/j.1432-1033.1976.tb10841.x.
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Photoaffinity labeling.
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Eur J Biochem. 1978 Apr 17;85(2):317-23. doi: 10.1111/j.1432-1033.1978.tb12241.x.

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