Tsuboyama A, Takaku F, Sakamoto S, Kano Y, Ariga T, Miyatake T
Br J Cancer. 1980 Dec;42(6):908-14. doi: 10.1038/bjc.1980.339.
Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance than-layer plate. The plate was developed and sprayed with resorcinol reagents and heated. Seventeen bands of gangliosides were demonstrated in normal human leucocytes. The composition of these gangliosides was different in the various kinds of leucocytes. Amounts of GM3 ganglioside were apparently greater in normal lymphocytes and leukaemic cells than in granulocytes. Among the acute-leukaemic cells, some kinds of complex gangliosides were much more abundant in myelogenous cells than in lymphocytic cells. These changes in ganglioside composition are suggested as new biochemical markers for leukaemic cells.
用氯仿 - 甲醇从白细胞沉淀中提取的脂质应用于二乙氨基乙基葡聚糖（DEAE - Sephadex）柱，神经节苷脂用甲醇中的0.2M醋酸钠洗脱。洗脱液通过葡聚糖G - 50柱色谱法脱盐。将纯化的神经节苷脂点样于高效薄层层析板上。展开薄板，喷间苯二酚试剂并加热。在正常人白细胞中显示出17条神经节苷脂条带。这些神经节苷脂的组成在各种白细胞中有所不同。正常淋巴细胞和白血病细胞中GM3神经节苷脂的含量明显高于粒细胞。在急性白血病细胞中，某些复杂神经节苷脂在髓细胞中比在淋巴细胞中丰富得多。神经节苷脂组成的这些变化被认为是白血病细胞的新生化标志物。
