Levy D, Hoang-Xuan M, Colombani M J, Zilber M T, Leclerc J C, Levy J P
J Immunol Methods. 1981;41(3):333-41. doi: 10.1016/0022-1759(81)90195-2.
A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary.
一种利用125I标记的蛋白A的细胞放射免疫测定法，用于检测附着在微量滴定板上的戊二醛固定细胞上的抗原-抗体复合物。该方法快速、灵敏且特异，可用于揭示H-2私有和公有特异性以及Ia和Lyt抗原。由于板可以保存数月，因此可以在一大组样本上一步测试多种反应性，无需定期供应动物。
