Warren M F, Hamilton P B
Poult Sci. 1981 Jan;60(1):120-3. doi: 10.3382/ps.0600120.
Day-old broiler chickens fed graded concentrations of ochratoxin A (0, .5, 1.0, 2.0, 4.0, and 8.0 micrograms/g of diet) for 3 weeks were examined histopathologically for glycogen accumulation in muscle tissue using both formalin and ethanol fixatives with periodic acid-Schiff stain in a duplicate diastatic slide technique. However, glycogen could not be visualized in chicken muscle by these techniques which work with human muscle. Quantitative analysis for the glycogen itself permitted demonstration of a significant (P less than .05) fourfold increase in birds fed 8 micrograms/g. Using startled birds demonstrated that this accumulated glycogen was about 99% physiologically available. These findings coupled with prior reports permit the assignment on morphologic criteria of the hyperglycogenation of ochratoxicosis as a type X glycogen storage disease.
给1日龄的肉鸡喂食不同浓度（0、0.5、1.0、2.0、4.0和8.0微克/克日粮）的赭曲霉毒素A，持续3周，然后使用福尔马林和乙醇固定剂，通过重复的淀粉酶切片技术，用高碘酸-希夫染色对肌肉组织中的糖原积累进行组织病理学检查。然而，这些适用于人类肌肉的技术无法在鸡肌肉中观察到糖原。对糖原本身的定量分析表明，喂食8微克/克赭曲霉毒素A的鸡体内糖原显著增加了四倍（P<0.05）。对受到惊吓的鸡的研究表明，这种积累的糖原约99%在生理上是可用的。这些发现与先前的报告相结合，使得根据形态学标准将赭曲霉毒素中毒的高糖原血症归类为X型糖原贮积病成为可能。
