关于从P群梭菌C48 - 50株（ATCC 29733）生产12α - 羟基类固醇脱氢酶的研究
On the production of 12 alpha-hydroxysteroid dehydrogenase from Clostridium group P, strain C48-50 ATCC 29733.
作者信息
Macdonald I A
出版信息
Experientia. 1981 May 15;37(5):451-2. doi: 10.1007/BF01986126.
PMID:6941897
Abstract
The production of 12 alpha-hydroxysteroid dehydrogenase of Clostridium group P strain C48-50 was optimized when the organism was grown in the presence of 2% fructose and 0.1% dithiothreitol. It appears that an initial redox potential of less than -160 mV (achieved by autoclaving in the presence of dithiothreitol, dithioerythritol or cysteine) is important in the production of this enzyme.
摘要
当P群梭菌C48 - 50菌株在2%果糖和0.1%二硫苏糖醇存在的条件下生长时，其12α - 羟基类固醇脱氢酶的产量得到优化。似乎初始氧化还原电位低于 - 160 mV（通过在二硫苏糖醇、二硫赤藓糖醇或半胱氨酸存在下高压灭菌实现）对该酶的产生很重要。
Zotero 插件