Herrera-Justiniano E, Recio F, Ferrer M, Díaz M, Villamil F, Aznar A
Rev Esp Fisiol. 1978 Jun;34(2):131-5.
The isolation of a human muscle extract that degrades insulin to products incapable of any immunological or biological activity is reported. The extract Km value for human insulin is 0.79, and 1.43 for porcine proinsulin. It does not degrade Human Growth Hormone at the concentrations tested, it is inactivated by N-ethyl-maleimide, Trasylol, and heat, and it does not require glutathione for its action. Human muscle, therefore can contain an enzyme similar to "insulin-specific-protease" present in rat muscle.
据报道，已从人体肌肉提取物中分离出一种能将胰岛素降解为无任何免疫活性或生物活性产物的物质。该提取物对人胰岛素的Km值为0.79，对猪胰岛素原的Km值为1.43。在所测试的浓度下，它不会降解人生长激素，会被N-乙基马来酰亚胺、抑肽酶和加热灭活，其作用不需要谷胱甘肽。因此，人体肌肉中可能含有一种类似于大鼠肌肉中存在的“胰岛素特异性蛋白酶”的酶。
