甲基汞（II）与单链和双链DNA的结合：通过磁共振光谱的光学检测可区分形成的复合物。
Methylmercury(II) binding to single-stranded and duplex DNA: complexes formed are distinguishable by optical detection of magnetic resonance spectroscopy.
作者信息
Maki A H, Ott C M
出版信息
Proc Natl Acad Sci U S A. 1981 May;78(5):2972-6. doi: 10.1073/pnas.78.5.2972.
Binding of CH3Hg(II) to duplex and single-stranded calf thymus DNA leads to an external heavy atom effect that is associated with the formation of complexes directly with the purine and pyrimidine bases. When CH3Hg(II) is added at a concentration insufficient to cause denaturation, clearly distinguishable optical detection of magnetic resonance spectra are observed from the duplex and single-stranded DNA complexes. Comparison of the dominant signals with those observed from CH3Hg(II) complexes of model mononucleotides and mononucleosides allows their identification as guanine complexed at N7 in the duplex sample and thymine complexed at N3 in the single-stranded sample. On the basis of these measurements, it is estimated that this experiment presently is capable of detecting about 2% single-stranded DNA in a sample made up predominantly after duplex structure.
CH3Hg(II)与双链和单链小牛胸腺DNA的结合会导致一种外部重原子效应，该效应与直接和嘌呤及嘧啶碱基形成复合物有关。当以不足以引起变性的浓度添加CH3Hg(II)时，从双链和单链DNA复合物中可观察到清晰可辨的磁共振光谱光学检测信号。将主要信号与从模型单核苷酸和单核苷的CH3Hg(II)复合物中观察到的信号进行比较，可将它们鉴定为双链样品中N7位络合的鸟嘌呤和单链样品中N3位络合的胸腺嘧啶。基于这些测量结果，据估计该实验目前能够在主要由双链结构组成的样品中检测到约2%的单链DNA。
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