Astaldi G, Bagnara G P, Biagini G, Baccarani M, Brunelli M A, Laschi R
Arch Geschwulstforsch. 1981;51(1):63-8.
Peripheral blood from 4 patients with chronic granulocytic leukemia during blast crisis has been investigated. One case has been studied before and after treatment with Arabinosyl-Cytosine and 6-Thioguanine. The nucleated blood cells have been separated by a discontinuous density gradient. Cells were obtained from six different gradient fractions (F1-F6: density ranging from 1.052 to 1.078). 0.5 X 10(5) cells from each density fraction have been cultured in agar culture system to evaluate the granulocyte-monocyte committed stem cells (Colony Forming Units-granulocyte monocyte: CFU-GM). Cells recovered from the same density fractions have been studied by electron microscopy to evaluate the number of less differentiated cells. A quantitative correlation between plating efficiency and blast cells number was carried out. The results indicate that the highest recovery of both CFU-GM and blast cells is present in light density fractions (specific density below 1.063). However a discrepancy between blast cell frequency and granulocyte-monocyte colony formation in the same density fractions appears to be evident. In the patient studied before and after treatment it appears that only one out of two light density fractions (F1) responds to the antiblastic treatment.
对4例慢性粒细胞白血病急变期患者的外周血进行了研究。其中1例在接受阿糖胞苷和6-硫鸟嘌呤治疗前后均进行了研究。有核血细胞通过不连续密度梯度进行分离。细胞取自六个不同的梯度级分（F1-F6：密度范围为1.052至1.078）。每个密度级分的0.5×10⁵个细胞已在琼脂培养系统中培养，以评估粒细胞-单核细胞定向干细胞（集落形成单位-粒细胞单核细胞：CFU-GM）。对从相同密度级分中回收的细胞进行了电子显微镜检查，以评估分化程度较低的细胞数量。对接种效率和原始细胞数量进行了定量相关性分析。结果表明，CFU-GM和原始细胞在低密度级分（比重低于1.063）中的回收率最高。然而，同一密度级分中原始细胞频率与粒细胞-单核细胞集落形成之间的差异似乎很明显。在治疗前后研究的患者中，似乎两个低密度级分（F1）中只有一个对抗白血病治疗有反应。
