Lollar P, Owen W G
Ann N Y Acad Sci. 1981;370:51-6. doi: 10.1111/j.1749-6632.1981.tb29720.x.
The effect of thrombin on release of adenine nucleotides is studied in cultured cell monolayers from the human umbilical vein. Thrombin-induced release of radioactivity from endothelial cells is dose-dependent and saturable with maximal response seen at 1 x 10(-8) M thrombin. The products are identified by thin-layer chromatography as adenine nucleotides. Diisopropylphosphoryl thrombin, which is enzymatically inactive, does not cause release of tritium. A 50-fold excess of diisopropylphosphoryl-thrombin, despite causing 98 percent inhibition of binding of 125I-thrombin to its high-affinity binding sites, does not inhibit thrombin-induced release. We conclude that (1) thrombin causes release from endothelial cells of adenine nucleotides and that (2) high-affinity, active-site-independent binding of thrombin is not involved in this process.
在人脐静脉培养的细胞单层中研究了凝血酶对腺嘌呤核苷酸释放的影响。凝血酶诱导内皮细胞释放放射性呈剂量依赖性且具有饱和性，在1×10⁻⁸ M凝血酶时可见最大反应。产物经薄层色谱法鉴定为腺嘌呤核苷酸。酶无活性的二异丙基磷酰凝血酶不会引起氚的释放。尽管50倍过量的二异丙基磷酰凝血酶可导致125I-凝血酶与其高亲和力结合位点的结合受到98%的抑制，但它并不抑制凝血酶诱导的释放。我们得出结论：（1）凝血酶导致内皮细胞释放腺嘌呤核苷酸；（2）此过程不涉及凝血酶的高亲和力、不依赖活性位点的结合。
