苯乙醇对大肠杆菌体外DNA合成的影响。

The effect of phenethyl alcohol on in vitro DNA synthesis in Escherichia coli.

作者信息

Kaneko M, Kodama M, Nagata C

出版信息

Biochim Biophys Acta. 1977 Jul 5;477(1):28-36. doi: 10.1016/0005-2787(77)90158-7.

Abstract

The effect of phenethyl alcohol on DNA synthesis was examined using several in vitro systems of Escherichia coli H560; i.e., ether-treated cells, membrane fractions and folded chromosomes fortified with DNA polymerase. In all systems, the incorporation of deoxyribonucleotides was much reduced for the phenethyl alcohol-treated cells compared with the non-treated cells. The total activity of DNA polymerases in polA1 cells (mostly DNA polymerase II) was not impaired for the phenethyl alcohol-treated cells and the reduction of the rate of DNA synthesis in vitro was ascribed to the reduction of the chromosomal template activity which was related to trypsin sensitive protein components. The analysis of chromosomes from the phenethyl alcohol-treated cells revealed the remarkable reduction of a protein component of molecular weight approx. 58 000 in contrast with a protein component of molecular weight approx. 30 000.

摘要

利用大肠杆菌H560的几种体外系统研究了苯乙醇对DNA合成的影响；即经乙醚处理的细胞、膜组分以及添加了DNA聚合酶的折叠染色体。在所有系统中，与未处理的细胞相比，经苯乙醇处理的细胞中脱氧核苷酸的掺入量大幅减少。对于经苯乙醇处理的细胞，polA1细胞（主要是DNA聚合酶II）中DNA聚合酶的总活性并未受损，体外DNA合成速率的降低归因于与胰蛋白酶敏感蛋白成分相关的染色体模板活性的降低。对经苯乙醇处理的细胞的染色体分析显示，分子量约为58000的一种蛋白成分显著减少，与之形成对比的是分子量约为30000的一种蛋白成分。