Wang B S, Onikul S R, Mannick J A
Science. 1978 Oct 6;202(4363):59-60. doi: 10.1126/science.694519.
The effect of immune RNA treatment on the incidence of death from pulmonary metastases was studied in C57BL/6J mice after excision of a B16 murine melanoma. Immune RNA was extracted from the lymphoid tissues of guinea pigs immunized with B16 tumor and then incubated in vitro with normal C57BL/6J mouse splenocytes. Mice receiving intraperitoneal injections of these RNA-treated syngeneic splenocytes after the primary B16 isograft was resectioned showed significantly improved long-term survival (42 to 67 percent in three successive experiments) as compared to control mice (0 to 20 percent survival) receiving untreated splenocytes. The effect of RNA treatment was tumor-specific and ribonuclease sensitive. The results suggest that immunotherapy with immune RNA may be of benefit to certain patients after surgery for cancer.
在切除B16小鼠黑色素瘤后，研究了免疫RNA治疗对C57BL/6J小鼠肺转移所致死亡率的影响。免疫RNA从用B16肿瘤免疫的豚鼠淋巴组织中提取，然后在体外与正常C57BL/6J小鼠脾细胞孵育。在初次B16同种异体移植瘤切除后，接受腹腔注射这些经RNA处理的同基因脾细胞的小鼠，与接受未处理脾细胞的对照小鼠（存活率0%至20%）相比，长期存活率显著提高（在连续三次实验中为42%至67%）。RNA治疗的效果具有肿瘤特异性且对核糖核酸酶敏感。结果表明，免疫RNA免疫疗法可能对某些癌症手术后的患者有益。
