Fish D C, Bare A L, Djurickovic D B, Huebner R J
J Natl Cancer Inst. 1981 Nov;67(5):1041-51.
F344 inbred were repeatedly immunized (days 0, 28, and 42) with normal syngeneic or allogeneic rat tissues or transplantable syngeneic or allogeneic rat tumors (some of which were virus producing). Immunized rats were challenged by sc injection of 10(5) or 10(6) syngeneic rat tumor cells from either of two different tumor lines. Successful cross-protective immunization prevented tumor development in rats that were challenged at 100-1,000 times the 50% tumor dose. The protection was essentially lifelong and complete in that no tumors appeared up to 200 days post challenge in some experiments. To be successful, the tumor cell vaccines had to express a complement-fixing cross-reacting antigen detected with sera from rats bearing any of several different tumors and to be able to induce a spontaneously regressing tumor in the host.
将F344近交系大鼠用正常同基因或异基因大鼠组织或可移植的同基因或异基因大鼠肿瘤（其中一些产生病毒）反复免疫（第0、28和42天）。用来自两种不同肿瘤系之一的10(5)或10(6)个同基因大鼠肿瘤细胞皮下注射对免疫大鼠进行攻击。成功的交叉保护性免疫可防止在以50%肿瘤剂量的100 - 1000倍进行攻击的大鼠中发生肿瘤。这种保护基本上是终身的且是完全的，因为在一些实验中，直到攻击后200天都没有出现肿瘤。为了成功，肿瘤细胞疫苗必须表达一种能用患有几种不同肿瘤之一的大鼠血清检测到的补体结合交叉反应抗原，并且能够在宿主体内诱导自发消退的肿瘤。
