Wall J R, Walters B A
Aust N Z J Med. 1981 Aug;11(4):375-9. doi: 10.1111/j.1445-5994.1981.tb03515.x.
Patients with lepromatous leprosy and tuberculoid leprosy, together with normal aged matched controls, were tested for in vitro immunoreactivity against a panel of soluble extracts prepared from normal human tissues. The panel consisted of a soluble homogenate of human testis as well as two partially purified fractions of this extract, and control extracts from other human tissues. Immunoreactivity was assessed by extract induced peripheral blood lymphocyte (PBL) transformation, leucocyte migration inhibition (LMI) and leucocyte adherence inhibition (LAI). Although no difference in PBL transformation, LMI, or LAI was obtained between the groups with the control tissue extracts, significant reactivity was obtained for the patients with lepromatous leprosy in at least one of the in vitro tests, when one of the testis extracts was used (Mann-Whitney test). No such reactivity to the testis extracts was obtained in the patients with tuberculoid leprosy or the normal control subjects. Of the twenty-two patients studied with lepromatous leprosy, thirteen had clinical evidence of testicular disease and nine of these patients had raised PBL transformation and LAI reactivity to the testis extracts. This finding of immunoreactivity against testicular extracts in a significant number of patients with lepromatous leprosy by at least one of the vitro tests used, suggests that the associated in vivo testicular atrophy that occurs in these patients may have an auto-immune basis.
对瘤型麻风病患者、结核样型麻风病患者以及年龄匹配的正常对照者，检测其针对一组由正常人体组织制备的可溶性提取物的体外免疫反应性。该组提取物包括人睾丸的可溶性匀浆及其两个部分纯化的组分，以及来自其他人体组织的对照提取物。通过提取物诱导的外周血淋巴细胞（PBL）转化、白细胞游走抑制（LMI）和白细胞黏附抑制（LAI）来评估免疫反应性。尽管在使用对照组织提取物的各组之间，PBL转化、LMI或LAI没有差异，但当使用其中一种睾丸提取物时，在至少一项体外试验中，瘤型麻风病患者获得了显著的反应性（曼-惠特尼检验）。结核样型麻风病患者或正常对照者对睾丸提取物未获得此类反应性。在研究的22例瘤型麻风病患者中，13例有睾丸疾病的临床证据，其中9例患者对睾丸提取物的PBL转化和LAI反应性升高。通过所使用的至少一项体外试验，在大量瘤型麻风病患者中发现对睾丸提取物的免疫反应性，这表明这些患者中发生的相关体内睾丸萎缩可能有自身免疫基础。
