Jones J G, Elmes M E
Scand J Gastroenterol Suppl. 1981;70:37-48.
The intracellular zinc concentration of human small intestinal mucosal cells was estimated using X-ray microanalysis. Jejunum and ileum from 11 patients resected during surgery was fixed in 2.5% glutaraldehyde and acetone and the estimations performed with an AEI EMMA 4. The zinc content of undifferentiated stem cells, enterocytes, goblet cells and Paneth cells was determined and all the cell types were found to contain significant amounts of zinc. The highest levels were found in the stem cells and enterocytes with a maximum in the ideal enterocytes in Crohn's disease. In non-inflammatory conditions the ileal enterocytes had a zinc content than the jejunal enterocytes. Stem cells had comparable levels in all tissues analysed. Goblet cells showed a wide variation in zinc levels that may be due to their secretory status. Paneth cells had the lowest zinc levels. The whole Paneth cell zinc and granule zinc were of the same order in all tissues sampled. The cytoplasmic zinc was higher than the granule zinc in all samples of ileum analysed, but was no significant difference in the jejunum. The limitations of the technique are considered and observations made on the role of zinc in intestinal mucosa.
采用X射线微量分析技术估算人小肠黏膜细胞内的锌浓度。取自11例手术切除患者的空肠和回肠，用2.5%戊二醛和丙酮固定，并用AEI EMMA 4进行估算。测定了未分化干细胞、肠上皮细胞、杯状细胞和潘氏细胞中的锌含量，发现所有细胞类型均含有大量锌。锌含量最高的是干细胞和肠上皮细胞，在克罗恩病的理想肠上皮细胞中达到最高值。在非炎症状态下，回肠肠上皮细胞的锌含量高于空肠肠上皮细胞。干细胞在所有分析组织中的锌含量相当。杯状细胞的锌含量差异较大，这可能与其分泌状态有关。潘氏细胞的锌含量最低。在所有采样组织中，整个潘氏细胞的锌含量和颗粒锌含量处于同一数量级。在所有分析的回肠样本中，细胞质锌含量高于颗粒锌，但在空肠中无显著差异。文中考虑了该技术的局限性，并对锌在肠黏膜中的作用进行了观察。
