铁蛋白的能量过滤透射电子显微镜观察
Energy-filtered transmission electron microscopy of ferritin.
作者信息
Shuman H, Somlyo A P
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(1):106-7. doi: 10.1073/pnas.79.1.106.
Abstract
The focusing properties of a magnetic-sector spectrometer are shown to be suitable for forming high-spatial-resolution, energy-filtered transmission electron microscope images. Filtered images of ferritin molecules by using electrons scattered from the characteristic iron M2,3 and carbon K absorption edges clearly distinguish the 75-A iron core and 120-A protein shell. The minimum detectable mass is estimated to be 0.84 X 10(-20) g for Fe for an electron dose of 18 C/cm2 and 99% confidence.
摘要
磁扇形光谱仪的聚焦特性被证明适用于形成高空间分辨率的能量过滤透射电子显微镜图像。通过使用从铁的特征M2,3和碳的K吸收边散射的电子获得的铁蛋白分子过滤图像，清晰地分辨出了75埃的铁芯和120埃的蛋白质外壳。对于18 C/cm2的电子剂量和99%的置信度，估计铁的最小可检测质量为0.84×10(-20)克。
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