Newman H N
J Periodontol. 1982 Feb;53(2):101-8. doi: 10.1902/jop.1982.53.2.101.
This study explores the relationship between calcium ions, matrix polymers and the formation of natural plaque on the children's teeth, using direct observation, scanning, transmission and freeze-etching electron microscopy techniques. Plaques were observed either in situ or after removal from enamel with a chelating agent, EDTA. Plaque was not disrupted by EDTA. Matrix polymers in outer plaque were less abundant than in deeper layers of specimens exposed to EDTA before embedding. The several cationic electron-histochemical reagents used generally disclosed more matrix than routine contrasting. The various polymers, other than discrete structures such as fimbriae, may derive from saliva and gingival fluid as well as bacteria. It is concluded that calcium ions are of importance in the early phase of bacterial attachment to the host organic enamel integument, but that calcium plays no part in the formation or maintenance of the strong bonds that link organisms in established plaque.
本研究运用直接观察、扫描、透射及冷冻蚀刻电子显微镜技术，探究钙离子、基质聚合物与儿童牙齿上天然菌斑形成之间的关系。菌斑既可以原位观察，也可以用螯合剂乙二胺四乙酸（EDTA）从牙釉质上移除后进行观察。EDTA不会破坏菌斑。外层菌斑中的基质聚合物比包埋前用EDTA处理的标本深层中的要少。所使用的几种阳离子电子组织化学试剂通常比常规对比法揭示出更多的基质。除了菌毛等离散结构外，各种聚合物可能来源于唾液、龈沟液以及细菌。研究得出结论，钙离子在细菌附着于宿主有机牙釉质覆盖物的早期阶段很重要，但在已形成菌斑中连接生物体的强键的形成或维持过程中，钙不起作用。
