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[从电子显微镜照片中加速测量浓缩染色质的体积]

[Accelerated measurement of the volume of condensed chromatin from electron microscopic photographs].

作者信息

Kukharenko N S, Stefanov S B

出版信息

Tsitologiia. 1978 Jul;20(7):815-7.

PMID:695009
Abstract

The fraction of condensed chromatin (C. C., %) in cell nuclei images was determined by the aid of the "random step grid". 100 random points were thrown on every electron microscopic images. The number of points in C. C. images was counted. Lymphocyte nuclei in spleen and lymph nodes of normal cows have a higher content of C. C. (61 +/- 1.6%) Than those of cows suffering from lympholeucosis in haematological (49 +/- 21%) and in tumor (44 +/- 1.1%) stages. The counting procedure takes about 2 hours for 90 images. The random step grid can be easily available in every day practice.

摘要

借助“随机步长网格”确定细胞核图像中凝聚染色质的比例(C.C.,%)。在每张电子显微镜图像上随机投放100个点。统计C.C.图像中的点数。正常奶牛脾脏和淋巴结中的淋巴细胞核C.C.含量较高(61±1.6%),高于处于血液学阶段(49±2.1%)和肿瘤阶段(44±1.1%)的淋巴细胞性白血病奶牛。对90张图像进行计数大约需要2小时。随机步长网格在日常实践中很容易获得。

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