Ferezou J, Rautureau J, Coste T, Gouffier E, Chevallier F
Am J Clin Nutr. 1982 Aug;36(2):235-44. doi: 10.1093/ajcn/36.2.235.
The kinetics of cholesterol labeling was studied in the plasma lipoproteins of three subjects who had received an oral dose of octadeuterated cholesterol and an intravenous administration of 3H-cholesterol and 14C-mevalonate or 13C-acetate. After each labeled cholesterol pulse into the plasma (absorption, exchange, or synthesis), the isotopic concentrations of free and esterified cholesterol became identical after 120 h. Before this time, very low-density lipoprotein free cholesterol appeared more easily exchangeable than high-density and low-density lipoprotein free cholesterol, high-density lipoproteins were shown to be a source for very low-density lipoprotein cholesterol esters and the role of very low-density lipoproteins associated with chylomicrons was demonstrated in the initial transport of dietary cholesterol. The rates of the various processes involved in cholesterol turnover were calculated. The total cholesterol inflow into the plasma by absorption and synthesis, determined by long-term kinetic data (18 or 28 wk) was consistent with the result obtained by sterol balance for the total cholesterol outflow from the plasma (fecal excretion and conversion into bile acids).
对三名受试者的血浆脂蛋白中胆固醇标记的动力学进行了研究，这些受试者口服了十八氘代胆固醇，并静脉注射了³H-胆固醇和¹⁴C-甲羟戊酸或¹³C-乙酸盐。在每次向血浆中注入标记胆固醇（吸收、交换或合成）后，游离胆固醇和酯化胆固醇的同位素浓度在120小时后变得相同。在此之前，极低密度脂蛋白游离胆固醇比高密度和低密度脂蛋白游离胆固醇更容易交换，高密度脂蛋白被证明是极低密度脂蛋白胆固醇酯的来源，并且在膳食胆固醇的初始转运中证明了与乳糜微粒相关的极低密度脂蛋白的作用。计算了胆固醇周转过程中各种过程的速率。通过长期动力学数据（18或28周）确定的通过吸收和合成进入血浆的总胆固醇流入量与通过血浆中总胆固醇流出（粪便排泄和转化为胆汁酸）的甾醇平衡获得的结果一致。
