人早幼粒细胞白血病细胞系HL60的生长模式。

Growth pattern of the human promyelocytic leukaemia cell line HL60.

作者信息

Foa P, Maiolo A T, Lombardi L, Toivonen H, Rytömaa T, Polli E E

出版信息

Cell Tissue Kinet. 1982 Jul;15(4):399-404. doi: 10.1111/j.1365-2184.1982.tb01057.x.

DOI:10.1111/j.1365-2184.1982.tb01057.x

Abstract

In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 10(6) cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 +/- 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 +/- 2.2 hr; S, 15.1 +/- 3 hr; and G2, 4 +/0 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the vitro study of substances which may affect human leukaemic myelopoietic proliferation.

摘要

为了描述人早幼粒细胞白血病细胞系HL60的生长模式，对其动力学参数进行了研究。通过连续细胞计数计算倍增时间，从标记有丝分裂曲线分析得出各个细胞周期阶段的持续时间，并通过连续标记实验确定静止细胞群。培养中的增殖呈指数增长，直至达到约3.0×10⁶个细胞/毫升的饱和密度，倍增时间为34.0小时。细胞周期持续时间为24.3±4.1小时（标准差），细胞周期各阶段的持续时间为：G1期，3.8±2.2小时；S期，15.1±3小时；G2期，4±1.2小时。生长分数为0.85，细胞损失仅限于静止细胞。具有充分表征动力学的HL60细胞系为体外研究可能影响人白血病骨髓增殖的物质提供了一个有用的工具。