Satyaswaroop P G, Wartell D J, Mortel R
Endocrinology. 1982 Sep;111(3):743-9. doi: 10.1210/endo-111-3-743.
Endometrial glands were separated from stromal cells by collagenase digestion of human endometrium, and the distribution of progesterone (P) receptor and the activities of P-regulated enzymes, 17 beta-estradiol dehydrogenase (E2DH) and 20 alpha-dihydroprogesterone dehydrogenase (20 alpha DH), were determined in these preparations. Concentrations of cytosolic P receptor were estimated by Scatchard plot analysis of specific [3H]P binding in intact proliferative endometrium and in glands and stromal cells isolated from this tissue. Epithelial cells were more than 10-fold enriched in high affinity, P-specific binding sites compared to stromal cells. The binding constants (Kd) for [3H]P binding were essentially similar in undissociated endometrium, glandular epithelium, and stroma, ranging from 1--5 nM. The activities of E2DH and 20 alpha DH were also 3-fold higher in the glandular epithelium than in whole tissue or stroma during both proliferative and secretory stages of the menstrual cycle. In addition, the induction of these enzyme activities by progestin in vitro in cultured explants of proliferative endometrium was restricted to the glandular epithelium. Thus, the effects of P on E2DH and 20 alpha DH are expressed in the same cells that contain receptors for P.
通过胶原酶消化人子宫内膜，将子宫内膜腺体与基质细胞分离，并在这些制剂中测定孕酮（P）受体的分布以及P调节酶17β-雌二醇脱氢酶（E2DH）和20α-二氢孕酮脱氢酶（20αDH）的活性。通过对完整增殖期子宫内膜以及从该组织分离的腺体和基质细胞中特异性[3H]P结合的Scatchard图分析，估计胞质P受体的浓度。与基质细胞相比，上皮细胞中高亲和力、P特异性结合位点的富集程度超过10倍。在未分离的子宫内膜、腺上皮和基质中，[3H]P结合的解离常数（Kd）基本相似，范围为1-5 nM。在月经周期的增殖期和分泌期，腺上皮中E2DH和20αDH的活性也比全组织或基质高3倍。此外，在体外培养的增殖期子宫内膜外植体中，孕激素对这些酶活性的诱导仅限于腺上皮。因此，P对E2DH和20αDH的作用在含有P受体的相同细胞中表达。
