McCune C S, O'Donnell R W, Horan P K, Budd H S, Spennacchio J L, Chuang C, Henshaw E C
J Natl Cancer Inst. 1982 Sep;69(3):647-52.
Hybrid tumor cells have been evaluated for their ability to induce specific antitumor immunity in inbred female C3H/He mice challenged with the syngeneic BA tumor. Hybrid cells were produced by fusion of BA cells with a BALB/c renal adenocarcinoma, which is hypoxanthine phosphoribosyl transferase-deficient and grows well in culture. Corynebacterium parvum was evaluated as an adjuvant for BA and hybrid cells. The BA tumor was shown to be poorly immunogenic, and four weekly injections of BA cells alone or C. parvum alone did not confer significant immunity. When BA cells and C. parvum were mixed, survival time was prolonged and most mice remained tumor-free. Hybrid cell lines derived from the BA tumor were produced in culture in unlimited quantities and were successfully used as immunogens. The addition of C. parvum to hybrids gave a significant incremental increase in survival when compared to the survival resulting from immunization by hybrids without adjuvant. When hybrids without adjuvant were used, several weekly injections were required for effective immunization. Irradiated and unirradiated hybrids were compared, and it was found that irradiation did not diminish hybrid immunogenicity. The potential problems and advantages of this concept of therapy are discussed.
在同基因BA肿瘤攻击的近交系雌性C3H/He小鼠中，对杂交肿瘤细胞诱导特异性抗肿瘤免疫的能力进行了评估。杂交细胞是通过将BA细胞与BALB/c肾腺癌融合产生的，该肾腺癌缺乏次黄嘌呤磷酸核糖基转移酶，在培养中生长良好。将短小棒状杆菌评估为BA细胞和杂交细胞的佐剂。结果显示BA肿瘤的免疫原性较差，单独每周注射4次BA细胞或单独注射短小棒状杆菌均未赋予显著的免疫力。当BA细胞和短小棒状杆菌混合时，存活时间延长，大多数小鼠保持无瘤状态。源自BA肿瘤的杂交细胞系在培养物中可大量产生，并成功用作免疫原。与未加佐剂的杂交细胞免疫相比，向杂交细胞中添加短小棒状杆菌可显著提高存活率。当使用未加佐剂的杂交细胞时，需要每周注射几次才能有效免疫。对经辐照和未辐照的杂交细胞进行了比较，发现辐照并未降低杂交细胞的免疫原性。讨论了这种治疗概念的潜在问题和优点。
