Koizumi S, Yamagami M, Miura M, Horita S, Sano M, Ikuta N, Taniguchi N
Blood. 1982 Oct;60(4):1046-9.
Expression of Ia antigens on granulocyte/macrophage colony-forming cells (CFU-GM) in human cord blood was compared with that in bone marrow with the use of monoclonal OKIal antibody. Mononuclear cells prepared from cord blood and bone marrow were pretreated with OKIal antibody plus complement, and, thereafter, the ability of cord blood and bone marrow cells to form colonies of CFU-GM was assayed in semisolid agar culture. Consistent reduction in the number of CFU-GM in cord blood to 58.8% +/- 13.0% (mean +/- SD) of controls treated with complement alone was shown after elimination of Ia-antigen-bearing CFU-GM, but was significantly remarkable than that in bone marrow (18.0% +/- 5.6%). Although the reduction of both granulocyte (CFC-G) and macrophage colony (CFC-M) types of cord blood, characterized by the double staining for esterase activity, was shown following treatment with OKIal antibody plus complement, the relative inhibition of CFC-G weas significantly greater than that of CFC-M (p less than 0.02). These results suggest some differences in the characteristics of Ia-antigen-bearing CFU-GM between cord blood and bone marrow cells. Furthermore, it is suggested that Ia-dependent regulatory mechanisms might participate in the differentiation of CFU-GM to CFC-G and CFC-M.
使用单克隆OKIal抗体，比较了人脐血中粒细胞/巨噬细胞集落形成细胞（CFU-GM）与骨髓中Ia抗原的表达情况。将从脐血和骨髓中制备的单个核细胞用OKIal抗体加补体进行预处理，然后在半固体琼脂培养中检测脐血和骨髓细胞形成CFU-GM集落的能力。去除携带Ia抗原的CFU-GM后，脐血中CFU-GM的数量持续减少，降至仅用补体处理的对照的58.8%±13.0%（平均值±标准差），但显著高于骨髓中的减少率（18.0%±5.6%）。虽然在用OKIal抗体加补体处理后，脐血中粒细胞（CFC-G）和巨噬细胞集落（CFC-M）类型均有减少，通过酯酶活性双重染色得以体现，但CFC-G的相对抑制作用显著大于CFC-M（p<0.02）。这些结果表明，脐血细胞与骨髓细胞中携带Ia抗原的CFU-GM的特征存在一些差异。此外，提示Ia依赖的调节机制可能参与了CFU-GM向CFC-G和CFC-M的分化过程。
