Brattain M G, Brattain D E, Sarrif A M, McRae L J, Fine W D, Hawkins J G
J Natl Cancer Inst. 1982 Oct;69(4):767-71.
The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency by cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.
测试了C3H/10T 1/2细胞饲养层对人和小鼠细胞系生长的影响。与在无饲养层的半固体培养基中生长的对照相比，饲养层增加了培养的癌细胞在半固体培养基中的集落形成。使用饲养层时，也能以更快的速度达到最大集落形成。当饲养细胞作为汇合单层存在时，直接从异种移植获得的癌细胞在组织培养中的接种效率提高了三倍至五倍。
