Daene A, Coutelle C, Thierbach V, Holle M, Raderecht C, Roigas H
Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(2):193-201.
A test for the cellular RNA-synthesis (incorporation of 3H-uridine in the RNA) of human bone marrow has been standardized with respect to the time of incorporation, the number of cells and the concentration of 3H-uridine. The following parameters were estimated for 500 microleter standard assay and 100 microleter aliquots for the determination of the radioactivity: time of incubation 80 min, number of nucleated cells 8 - 10(5), concentration of 3H-uridine 8,3 - 10(-6) M. Actinomycin D inhibits the RNA-synthesis to 90% in a concentration of 1.2 - 10(2) microgram/ml. The test appears generally applicable for the determination of the vitality of bone marrow after cryopreservation, the testing of cryoprotectants and haematotoxic substances and the control of the reaction of the bone marrow during chemical- or irradiation treatment of tumors.
一项关于人类骨髓细胞RNA合成（3H-尿苷掺入RNA）的检测方法，已在掺入时间、细胞数量和3H-尿苷浓度方面实现了标准化。针对用于放射性测定的500微升标准检测和100微升等分试样，估算了以下参数：孵育时间80分钟，有核细胞数量8 - 10(5)，3H-尿苷浓度8.3 - 10(-6) M。放线菌素D在浓度为1.2 - 10(2)微克/毫升时可将RNA合成抑制90%。该检测方法通常适用于测定冷冻保存后骨髓的活力、检测冷冻保护剂和血液毒性物质，以及在肿瘤的化学或放射治疗过程中控制骨髓的反应。
