Kreicbergs A, Broström L A, Cewrien G, Einhorn S
Cancer. 1982 Dec 1;50(11):2476-81. doi: 10.1002/1097-0142(19821201)50:11<2476::aid-cncr2820501136>3.0.co;2-n.
The cellular DNA content was determined in a consecutive series of 26 classical and two paraosteal osteosarcomas by means of single cell cytophotometry. All classical osteosarcomas regardless of histologic type (osteo-, chondro-, fibroblastic) or grade were found to be hyperploid (abnormally increased DNA content) while both paraosteal osteosarcomas proved to be diploid (normal DNA content). Closer analysis of the DNA distribution curves showed that the majority of the classical osteosarcomas exhibited a triploid modal DNA value and more than 90% hyperploid cells. Cytochemical characterization of osteosarcomas may be of prognostic significance in reflecting the degree of malignancy. The results of the present study indicate that cytophotometric DNA analysis, as an adjunct to conventional histologic evaluation, offers an objective means of differentiating between classical and paraosteal osteosarcomas.
通过单细胞细胞光度测定法，对连续的26例经典型骨肉瘤和2例骨旁骨肉瘤进行了细胞DNA含量测定。结果发现，所有经典型骨肉瘤，无论其组织学类型（骨母细胞型、软骨母细胞型、纤维母细胞型）或分级如何，均为超二倍体（DNA含量异常增加），而2例骨旁骨肉瘤均为二倍体（DNA含量正常）。对DNA分布曲线的进一步分析表明，大多数经典型骨肉瘤呈现三倍体的DNA众数，且超二倍体细胞超过90%。骨肉瘤的细胞化学特征在反映恶性程度方面可能具有预后意义。本研究结果表明，细胞光度法DNA分析作为传统组织学评估的辅助手段，为区分经典型骨肉瘤和骨旁骨肉瘤提供了一种客观方法。
