Slavkin H C, Zeichner-David M, MacDougall M, Bessem C, Bringas P, Honig L S, Lussky J, Vides J
J Dent Res. 1982 Dec;Spec No:1467-71.
Epithelial-mesenchymal interactions regulate determination and differentiation of amelogenesis. Our attention has focused on identification of ameloblast gene products, the regulation of enamel mRNA synthesis, and subsequent translation into enamel proteins in vivo and in vitro. Enamel proteins are the most abundant gene products synthesized in fully-differentiated ameloblasts. Our experimental strategy has been to isolate major proteins, produce antibodies, localize enamel protein antigens during tooth development in vivo as well as in vitro (using serumless, chemically-defined medium), develop an immunoprecipitation assay, isolate poly(A)-products in a cell-free translation system, and then initiate molecular cloning of the corresponding murine enamel gene(s). The major murine enamel mRNA appears to code for a predominant polypeptide of approximately 20,000 MW. Inner-enamel epithelial cells differentiate into ameloblasts, and synthesize and secrete enamel proteins within six d when cap-stage molar tooth organs are cultured in serumless, chemically-defined medium. The regulation of epithelial differentiation under these experimental conditions indicates that epithelial-mesenchymal interactions determine and maintian ameloblast differentiation in vitro.
上皮-间充质相互作用调节釉质形成的决定和分化。我们的注意力集中在成釉细胞基因产物的鉴定、釉质mRNA合成的调节以及随后在体内和体外将其翻译成釉质蛋白。釉质蛋白是在完全分化的成釉细胞中合成的最丰富的基因产物。我们的实验策略是分离主要蛋白质、制备抗体、在体内和体外（使用无血清、化学成分确定的培养基）牙齿发育过程中定位釉质蛋白抗原、开发免疫沉淀测定法、在无细胞翻译系统中分离聚腺苷酸产物，然后启动相应小鼠釉质基因的分子克隆。主要的小鼠釉质mRNA似乎编码一种分子量约为20,000的主要多肽。当帽状期磨牙器官在无血清、化学成分确定的培养基中培养时，内釉上皮细胞在6天内分化为成釉细胞，并合成和分泌釉质蛋白。这些实验条件下上皮分化的调节表明，上皮-间充质相互作用在体外决定并维持成釉细胞的分化。
