Davis T P, Peng Y M, Goodman G E, Alberts D S
J Chromatogr Sci. 1982 Nov;20(11):511-6. doi: 10.1093/chromsci/20.11.511.
High performance liquid chromatographic procedures are described for melphalan, bisantrene, and 13-cis retinoic acid, three important anticancer drugs in various stages of clinical development. The procedures require a rapid and simple sample clean-up followed by a 10-to 20-min chromatographic separation on a reversed-phase C18 column. Precisions are all less than 8% with recoveries greater than 80%. Mass spectrometry confirmation of each drug from patient sample separations is presented to provide unambiguous identification for valid pharmacokinetic parameter determination.
描述了用于美法仑、比生群和13-顺式维甲酸这三种处于临床开发不同阶段的重要抗癌药物的高效液相色谱方法。这些方法要求对样品进行快速简单的净化，然后在反相C18柱上进行10至20分钟的色谱分离。精密度均小于8%，回收率大于80%。还展示了对患者样品分离得到的每种药物进行质谱确认，以便为有效药代动力学参数测定提供明确的鉴定。
